Assessment of the synergistic effect of a poly-herbals combination on the antioxidant activity through a statistical approach

Poly-herbals combination was applied to enhance biological activity, particularly antioxidant activity. This present study purposed assess the synergistic interaction of a five traditional Indonesian herbal plants through simultaneous and integrated statistical technique. The were extracted using maceration, purification conducted before extraction eliminate ballast compounds. A simplex lattice design comprising 35 points utilized understand combinations' main effect multiple linear regression analysis on activity DPPH ABTS assays. results showed that C. longa, P. niruri, xanthorrhiza had most potent than M. oleifera asiatica. presence longa modulated between combinations. Meanwhile, non-curcuminoid content in played fundamental role reducing could poly-herbals combination. In addition, particular consideration should be withdrawn by antagonism for activity.